Glycolate Formation in Intact Spinach Chloroplasts
نویسندگان
چکیده
منابع مشابه
Mechanism of glycolate transport in spinach leaf chloroplasts.
The incorporation of (14)CO(2) into glycolate by intact spinach leaf (Spinacia oleracea L. var. Kyoho) chloroplasts exposed to (14)CO(2) (NaH(14)CO(3), 1 millimolar) in the light was determined as a function of O(2) concentrations in the reaction media. A hyperbolic saturation curve was obtained, apparent K(m) (O(2)) of 0.28 millimolar, indicating that glycolate is produced predominantly by rib...
متن کاملPhotosynthetic enhancement studied in intact spinach chloroplasts.
The Emerson enhancement effect was evaluated in the intact spinach (Spinacia oleracea var. Long Standing Bloomsdale) chloroplast by monitoring the uptake of (14)CO(2) during illumination by 640 nm and 720 nm lights. Low levels (about 10 mum) of fructose 1,6-diphosphate, ribose 5-phosphate, and glycerate 3-phosphate stimulated the rate of photosynthesis and abolished enhancement values observed ...
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Glycidate (2,3-epoxypropionate) increased CO(2) photoassimilation in intact spinach (Spinacia oleracea L.) chloroplasts in the presence of various inhibitors of photosynthesis, including O(2), arsenite, azide, iodo-acetamide, and carbonylcyanide 3-chlorophenylhydrazone. Although the mechanism by which glycidate enhances photosynthesis is obscure, the stimulatory effect cannot be ascribed to eit...
متن کاملInfluence of pH upon the Warburg Effect in Isolated Intact Spinach Chloroplasts: I. Carbon Dioxide Photoassimilation and Glycolate Synthesis.
The influence of pH upon the O(2) inhibition of (14)CO(2) photoassimilation (Warburg effect) was examined in intact spinach (Spinacia oleracea) chloroplasts. With conditions which favored the Warburg effect, i.e. rate-limiting CO(2) and 100% O(2), O(2) inhibition was greater at pH 8.4 to 8.5 than at pH 7.5 to 7.8. At pH 8.5, as compared with 7.8, there was an enhanced (14)C-labeling of glycolat...
متن کاملCharacterization of elemental sulfur in isolated intact spinach chloroplasts.
Incubation of intact spinach (Spinacia oleracea L.) chloroplasts in the presence of (35)SO(4) (2-) resulted in the light-dependent formation of a chloroform-soluble sulfur-containing compound distinct from sulfolipid. We have identified this compound as the most stable form (S(8)) of elemental sulfur (S(0), valence state for S = O) by mass spectrometry. It is possible that elemental sulfur (S(0...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1970
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.45.4.470